The evaluation of intensity of formation of biomembrane by microscopic fungi of the Candida genus

N. Sachivkina, E. Lenchenko, A. Strizakov, V. Zimina, L. Gnezdilova, V. Gavrilov, V. Byakhova, S. Germanova, A. Zharov, M. A. Molchanova

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25 Citations (Scopus)

Abstract

In the modern world, one of the most serious problems in the treatment of chronic infectious diseases of animals is the creation of biomembranes by microorganisms, in which they become inaccessible to the vast majority of antimicrobial agents. In this study, we investigated the structural and functional features of three strains of Candida albicans, Candida tropicalis and Candida africana on the ability to form biomembranes. A reliable diagnostic algorithm for quantitative evaluation of Candida biomembranes and remaining native cells has been tested. The dependence of the biomembrane formation process and the number of remaining native cells on the time of cultivation was determined. Morphological studies of the process of biomembrane formation without architectonics disturbance with the use of scanning electron microscope and cell holographic microscope were carried out.

Original languageEnglish
Pages (from-to)738-744
Number of pages7
JournalInternational Journal of Pharmaceutical Research
Volume10
Issue number4
DOIs
Publication statusPublished - 1 Oct 2018

Keywords

  • Candida africana
  • Candida albicans
  • Candida tropicalis
  • Cell holographic microscopy
  • Matrix
  • Microscopic fungi
  • Scanning electron microscopy

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